Human Phospho-IRS1 (Y1179) Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators.  Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2.  Human IRS1 is 1242 amino acids (aa) in length.  It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225).  IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Insulin receptor-mediated phosphorylation of human IRS1 on Y1179, or its murine equivalent, Y1172, mediates IRS-1 interaction with SH2 domains such as that of the Src family kinase, Fyn, or the tyrosine phosphatase, SHP-2.

Product Datasheets

Related Research Areas

• Adaptor Proteins
• Adaptor Proteins in the Akt Pathway
• Common gamma Chain Receptor Family
• Diabetes
• Endocrine Regulation of Lipid Metabolism
• GDNF Family
• Glucose Homeostasis
• Growth Hormone/IGF-I Axis
• IL-10 Family
• Interferons
• Metabolic Peptide Hormones and Regulators
• Nutrition and Appetite
• phospho-Serine/phospho-Threonine Binding Proteins