Human phospho-IRS1 (Y1179) Antibody (744353)

Name: Human phospho-IRS1 (Y1179) Antibody (744353)
Brand: R&D Systems
SKU: MAB7455
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB7455

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 744353

View all IRS1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human IRS1 Y1179 site

Specificity

Detects Human Phospho-IRS1 (Y1179) in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human phospho-IRS1 (Y1179) Antibody (744353)

Detection of Human Phospho-IRS1 (Y1179) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µg/mL Insulin for 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-IRS1 (Y1179) Monoclonal Antibody (Catalog # MAB7455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-IRS1 (Y1179) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-IRS1 (Y1179) in MCF‑7 Human Cell Line.

IRS1 phosphorylated at Y1179 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-IRS1 (Y1179) Monoclonal Antibody (Catalog # MAB7455) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human phospho-IRS1 (Y1179) Antibody (744353)

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Insulin and CIP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Human IRS1 is 1242 amino acids (aa) in length. It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Insulin receptor-mediated phosphorylation of human IRS1 on Y1179, or its murine equivalent, Y1172, mediates IRS-1 interaction with SH2 domains such as that of the Src family kinase, Fyn, or the tyrosine phosphatase, SHP-2.