Human Phospho-MLKL (T357) Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB9187S Datasheet / COA / SDS

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FAB9187S-100UG

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Human Phospho-MLKL (T357) Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MLKL when phosphorylated at T357.

Source

Monoclonal Mouse IgG2a Clone # 954702

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human MLKL T357 site
Accession # Q8NB16

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MLKL

Mixed Lineage Kinase Domain-Like (MLKL) is a 471 amino acid member of the protein kinase superfamily, but lacks several amino acid residues necessary for kinase activity. Phosphorylation of MLKL at T357 and S358 by receptor-interacting protein 3 (RIP3) causes trimerization and translocation of MLKL from the cytosol to the plasma membrane, where it mediates TNF-alpha induced necroptosis through membrane degradation and calcium influx. Low expression of MLKL has been found to be correlated with poor prognosis in cervical squamous cell carcinoma, pancreatic cancer, and ovarian cancer. Conversely, high expression of RIPK3 and MLKL in esophageal and colon cancer is associated with poor overall survival, possibly by promoting inflammatory cytokine production, sustaining tumor growth.

Long Name

Mixed Lineage Kinase Domain-like

Entrez Gene IDs

197259 (Human); 74568 (Mouse); 690743 (Rat)

Alternate Names

FLJ34389; mixed lineage kinase domain-like protein; mixed lineage kinase domain-like; MLKL