Human Phospho-STAT5a/b (Y694/Y699) Antibody

Catalog # Availability Size / Price Qty
Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot.
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Human Phospho-STAT5a/b (Y694/Y699) Antibody Summary

Species Reactivity
Detects human STAT5a/b when phosphorylated at Y694/Y699 in Western blots.
Monoclonal Rat IgG2A Clone # 941212
Protein A or G purified from cell culture supernatant
Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694).
Accession # P51692
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
0.3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-STAT5a/b (Y694/Y699) antibody by Western Blot. View Larger

Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT5a/b (Y694/Y699) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-STAT5a/b (Y694/Y699) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-STAT5a/b (Y694/Y699) in HeLa Human Cell Line. STAT5a/b phosphorylated at Y694/Y699 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5a/b

Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN alpha ). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN3GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Long Name
Signal Transducer and Activator of Transcription 5b
Alternate Names
MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b

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Human Phospho-STAT5a/b (Y694/Y699) Antibody
By Anonymous on 07/04/2022
Application: ICC/IF Sample Tested: HeLa human cervical epithelial carcinoma cell line Species: Human