Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Bat

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 6318100
View all PLZF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLZF
Met1-Gln254
Accession # Q05516

Specificity

Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PLZF APC‑conjugated Antibody

Detection of PLZF antibody in HL-60 Human Cell Line antibody by Flow Cytometry.

Detection of PLZF in HL‑60 Human Cell Line by Flow Cytometry.

HL-60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human PLZF APC-conjugated Monoclonal Antibody (Catalog # IC2944A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human PLZF APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HL‑60 human acute promyelocytic leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PLZF

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Long Name

Promyelocytic Leukemia Zinc Finger

Alternate Names

ZBTB16, ZNF145

Entrez Gene IDs

7704 (Human); 235320 (Mouse)

Gene Symbol

ZBTB16

UniProt

Q05516

Additional PLZF Products

Product Documents for Human PLZF APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PLZF APC‑conjugated Antibody

For research use only

Citations for Human PLZF APC‑conjugated Antibody

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