Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1067146
View all PMEL Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human PMEL
Lys25-Gln595
Accession # P40967

Specificity

Detects human PMEL in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PMEL Antibody

Detection of Human PMEL by Western Blot.

Western Blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PMEL at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of PMEL in SK-MEL-28 cells (Positive) and K562 cells (Negative).

PMEL was detected in fixed SK‑Mel‑28 human malignant melanoma cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative) using Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11470) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PMEL Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Fixed SK-Mel-28 human malignant melanoma cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative)

Western Blot

2 µg/mL
Sample: A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PMEL

Premelanasome protein (PMEL) is a nonmutated "self" antigen expressed by melanocytes, most melanoma cells, substantia nigra, and retinal pigmented epithelium.  This protein plays an essential role in the structural organization of premelanosomes. PMEL is over-expressed in all stages of melanoma progression and is negative prognostic marker for the overall survival rate of melanoma patients.  PMEL is also associated with melanin-based pigmentation, neurodegenerative disease (functional amyloid) and glaucoma (missense mutations). How PMEL amyloid is tolerated within cells is of major therapeutic interest as it is related to Alzheimer's and Parkinson's disease.

References

  1. Zhang, S. Chen, K. Liu, H. Jing, C. Zhang, X. Qu, C. Yu, S. PMEL as a Prognostic Biomarker and Negatively Associated With Immune Infiltration in Skin Cutaneous Melanoma (SKCM). J. Immunother. 2021 Jul-Aug 01;44(6):214-223.
    doi: 10.1097/CJI.0000000000000374. PMID: 34028390; PMCID: PMC8225232.

  2. Fan P.-D. Goff S.P. Mol. Cell. Biol. 20:7591-7601(2000). Chrystal, P.W. Footz, T. Hodges, E.D. Jensen, J.A. Walter, M.A. Allison, W.T. Functional Domains and Evolutionary History of the PMEL and GPNMB Family Proteins. Molecules. 2021 Jun 9;26(12):3529.
    doi: 10.3390/molecules26123529. PMID: 34207849; PMCID: PMC8273697.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Entrez Gene IDs

6490 (Human); 20431 (Mouse)

Gene Symbol

PMEL

UniProt

P40967 (Human)

Additional PMEL Products

Product Documents for Human PMEL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PMEL Antibody

For research use only

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Protocols

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