Human PMEL Antibody

Catalog #: MAB11470 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11470-100
MAB11470-SP

Save 15% on Select RUO Reagents. See Details.

Detection of Human PMEL by Western Blot.

2 Images

All PMEL Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PMEL Antibody Summary

Species Reactivity

Human

Specificity

Detects human PMEL in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1067146

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human PMEL
Lys25-Gln595
Accession # P40967

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

A549 human lung carcinoma cell line

Immunocytochemistry

3-25 µg/mL

Fixed SK-Mel-28 human malignant melanoma cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human PMEL by Western Blot. Western Blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PMEL at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry/ Immunofluorescence

View Larger

Detection of PMEL in SK-MEL-28 cells (Positive) and K562 cells (Negative). PMEL was detected in fixed SK‑Mel‑28 human malignant melanoma cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative) using Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11470) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PMEL

Premelanasome protein (PMEL) is a nonmutated "self" antigen expressed by melanocytes, most melanoma cells, substantia nigra, and retinal pigmented epithelium. This protein plays an essential role in the structural organization of premelanosomes. PMEL is over-expressed in all stages of melanoma progression and is negative prognostic marker for the overall survival rate of melanoma patients. PMEL is also associated with melanin-based pigmentation, neurodegenerative disease (functional amyloid) and glaucoma (missense mutations). How PMEL amyloid is tolerated within cells is of major therapeutic interest as it is related to Alzheimer's and Parkinson's disease.

References

Zhang, S. Chen, K. Liu, H. Jing, C. Zhang, X. Qu, C. Yu, S. PMEL as a Prognostic Biomarker and Negatively Associated With Immune Infiltration in Skin Cutaneous Melanoma (SKCM). J. Immunother. 2021 Jul-Aug 01;44(6):214-223.
doi: 10.1097/CJI.0000000000000374. PMID: 34028390; PMCID: PMC8225232.
Fan P.-D. Goff S.P. Mol. Cell. Biol. 20:7591-7601(2000). Chrystal, P.W. Footz, T. Hodges, E.D. Jensen, J.A. Walter, M.A. Allison, W.T. Functional Domains and Evolutionary History of the PMEL and GPNMB Family Proteins. Molecules. 2021 Jun 9;26(12):3529.
doi: 10.3390/molecules26123529. PMID: 34207849; PMCID: PMC8273697.

Long Name

Premelanosome Protein

Entrez Gene IDs

6490 (Human); 20431 (Mouse)

Alternate Names

D12S53EP1; Gp100; ME20; ME20M; ME20-M; melanocyte protein mel 17; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; PMEL; PMEL17; PMEL17P100; premelanosome proteinME20M; SI; SIL; SILV; silver (mouse homolog) like; silver homolog (mouse); Silver locus protein homolog; silver, mouse, homolog of; SILVPmel17