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Human Porimin Antibody

Catalog #: MAB3010
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3010
MAB3010-SP

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Detection of Porimin in MDA-MB-231 cells by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Porimin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Porimin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 297617
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Porimin
His27-Ser149
Accession # AAG21694
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Porimin
Flow Cytometry
2.5 µg/106 cells
Jurkat human acute T cell leukemia cell line, MDA‑MB‑231 human breast cancer cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Porimin in MDA-MB-231 cells by Flow Cytometry. MDA-MB-231 cells were stained with Mouse Anti-Human Porimin Monoclonal Antibody (Catalog # MAB3010, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Porimin

Porimin is a widely expressed mucin-related transmembrane glycoprotein. Cross-linking of Porimin leads to membrane disruption and cell death.

Long Name
Pro-Oncosis Receptor Inducing Membrane Injury
Entrez Gene IDs
114908 (Human)
Alternate Names
KCT3; KCT-3; KCT3keratinocytes associated transmembrane protein 3; Keratinocytes-associated transmembrane protein 3; Porimin; PORIMINporimin; pro oncosis receptor inducing membrane injury; Pro-oncosis receptor inducing membrane injury; serine/threonine-rich receptor; TMEM123; transmembrane protein 123PORMIN

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Citations for Human Porimin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TMEM123 a key player in immune surveillance of colorectal cancer
    Authors: Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara et al.
    Frontiers in Immunology
  2. TMEM123 a key player in immune surveillance of colorectal cancer
    Authors: Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara et al.
    Frontiers in Immunology
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry

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