Human POU2F3 Antibody (1091214) [Alexa Fluor™ Plus 488]

R&D Systems | Catalog # FAB11625AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 1091214
View all POU2F3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide

Specificity

Detects a synthetic peptide specific for human POU2F3 around amino acid 100 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human POU2F3 Antibody (1091214) [Alexa Fluor™ Plus 488]

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: POU2F3

POU2F3 is a 47 kDa transcription factor involved in the regulation of the differentiation of keratinocytes. It is also a part of the POU2F3-POU2AF2/POU2AF3 complex that drives the expression of tuft-cell-specific genes. Normal and neoplastic tuft cells share a genetic requirement for POU2F3. POU2F3 is utilized as an ancillary marker for the diagnosis of neuroendocrine-low small cell lung cancer. It is also positive in mesenchymal and neuroectodermal tumors including synovial carcinoma, solitary fibrous tumor, glioblastoma, Wilms tumor and melanoma.

References

  1. Hildesheim J, Kühn U, Yee CL, Foster RA, Yancey KB, Vogel JC. The hSkn-1a POU transcription factor enhances epidermal stratification by promoting keratinocyte proliferation. J Cell Sci. 2001 May;114(Pt 10):1913-23. doi: 10.1242/jcs.114.10.1913. PMID: 11329378. 
  2. Wu XS, He XY, Ipsaro JJ, Huang YH, Preall JB, Ng D, Shue YT, Sage J, Egeblad M, Joshua-Tor L, Vakoc CR. OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineage. Nature. 2022 Jul;607(7917):169-175. doi: 10.1038/s41586-022-04842-7. Epub 2022 May 16. PMID: 35576971; PMCID: PMC9419707. 
  3. Kaczorowski M, Ylaya K, Chłopek M, Lasota J, Miettinen M. Expression of POU2F3 Transcription Factor and POU2AF2, POU2F3 Coactivator, in Tuft Cell-like Carcinoma and Other Tumors. Am J Surg Pathol. 2024 Sep 25. doi: 10.1097/PAS.0000000000002313. Epub ahead of print. PMID: 39319626.

Long Name

POU class 2 homeobox 3

Alternate Names

Epoc-1, OCT-11, OCT11, OTF-11, PLA-1, PLA1, Skn-1a

Entrez Gene IDs

25833 (Human); 18988 (Mouse); 116544 (Rat)

Gene Symbol

POU2F3

UniProt

Q9UKI9 (Mouse)

Additional POU2F3 Products

Product Documents for Human POU2F3 Antibody (1091214) [Alexa Fluor™ Plus 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human POU2F3 Antibody (1091214) [Alexa Fluor™ Plus 488]


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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