Human Progesterone R/NR3C3 Antibody

R&D Systems | Catalog # AF5415

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Confocal Microscopy, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Progesterone R/NR3C3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PR-B
Met1-Leu189
Accession # P06401

Specificity

Detects human PR-A and PR-B in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Progesterone R/NR3C3 Antibody

Detection of Human Progesterone R/NR3C3 antibody by Western Blot.

Detection of Human Progesterone R/NR3C3 by Western Blot.

Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Progesterone R/NR3C3 antibody in T47D Human Cell Line by Immunocytochemistry (ICC).

Progesterone R/NR3C3 in T47D Human Cell Line.

Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Progesterone R/NR3C3 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

Progesterone R/NR3C3 in Human Prostate Cancer Tissue.

Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Progesterone R/NR3C3 by Simple WesternTM.

Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Progesterone R/NR3C3 at approximately 122 kDa (as indicated) using 25 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Progesterone R/NR3C3 Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed T47D human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Simple Western

25 µg/mL
Sample: T47D human breast cancer cell line

Western Blot

1 µg/mL
Sample: T47D human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Progesterone R/NR3C3

Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region (aa 1‑566), a DNA binding domain (aa 567‑639), and a
steroid-binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 by ERK1/2. An alternate start site at Met165 generates 90 kDa
PR‑A, an isoform particularly important in the ovary and uterus that insures fertility. Other isoforms show a deletion of either aa 637‑738 or 598‑636, or a 17 aa substitution for aa 787‑933.

Long Name

Progesterone Receptor

Alternate Names

NR3C3, PGR, ProgesteroneR

Entrez Gene IDs

5241 (Human)

Gene Symbol

PGR

UniProt

P06401

Additional Progesterone R/NR3C3 Products

Product Documents for Human Progesterone R/NR3C3 Antibody

Certificate of Analysis

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Product Specific Notices for Human Progesterone R/NR3C3 Antibody

For research use only

Citations for Human Progesterone R/NR3C3 Antibody

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Protocols

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