Human Prostasin/Prss8 Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Prostasin/Prss8

Prostasin, also known as Prss8 and channel activating protease 1, is a 40 kDa serine protease with trypsin-like substrate specificity that is a member of the peptidase S1 family. The enzyme is synthesized from a 343 amino acid (aa) preproenzyme that contains a 29 aa signal peptide, a propeptide (aa 30-32) and a 290 aa mature chain. A second propeptide (aa 323-343) contains a 21 aa transmembrane region (GPI anchor) which can be proteolytically processed to generate a secreted form of the enzyme. The mature chain forms a heterodimer made up of a light chain (aa 33-44) and a heavy chain (aa 45-322) that are connected by disulfide bonds. In addition, aa 45-286 constitute a peptidase S1 domain, and aa 159 is a potential site for N-linked glycosylation. Mature human Prostasin is 79% and 78% aa identical to mature mouse and rat Prostasin, respectively. Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, salivary gland, and pancreas. It activates amiloride-sensitive sodium channels.

Product Datasheets

Related Research Areas

• Epithelial Cell Markers and Intracellular Molecules
• Search Bioprocessing Enzymes
• Serine Proteases and Regulators
• Surface Ectodermal Cells