Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Prostasin/Prss8 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Prostasin
Ala33-Gly319
Accession # Q16651

Specificity

Detects human Prostasin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse Prostasin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Prostasin/Prss8 Antibody

Detection of Human Prostasin/Prss8 antibody by Western Blot.

Detection of Human Prostasin/Prss8 by Western Blot.

Western blot shows lysates of human prostate tissue, LNCaP human prostate cancer cell line, PC-3 human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostasin/Prss8 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Prostasin/Prss8 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

Prostasin/Prss8 in Human Prostate Cancer Tissue.

Prostasin/Prss8 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 10 µg/mL Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Prostasin/Prss8 antibody by Simple WesternTM.

Detection of Human Prostasin/Prss8 by Simple WesternTM.

Simple Western lane view shows lysates of human prostate tissue and LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Prostasin/Prss8 at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Prostasin/Prss8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Prostasin/Prss8 (Catalog # 4599-SE), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: Human prostate tissue and LNCaP human prostate cancer cell line

Western Blot

1 µg/mL
Sample: human prostate tissue and LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prostasin/Prss8

Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of Prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of Prostasin is involved in the regulation of epithelial sodium channels (4, 5).

References

  1. Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
  2. Yu, J.X. et al. (1994) J. Biol. Chem. 269:18843.
  3. Yu, J.X. et al. (1995) J. Biol. Chem. 270:13483.
  4. Caldwell, R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C194.
  5. Tong, Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.

Alternate Names

Prss8

Entrez Gene IDs

5652 (Human); 76560 (Mouse)

Gene Symbol

PRSS8

UniProt

Q16651

Additional Prostasin/Prss8 Products

Product Documents for Human Prostasin/Prss8 Antibody

Certificate of Analysis

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Product Specific Notices for Human Prostasin/Prss8 Antibody

For research use only

Citations for Human Prostasin/Prss8 Antibody

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Protocols

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