Human PTPN14 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB4458X Datasheet / COA / SDS

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FAB4458X-100UG

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Human PTPN14 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human PTPN14 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PTPN13 or rhPTPN22.

Source

Monoclonal Mouse IgG2b Clone # 448701

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PTPN14
Leu329-Leu900
Accession # Q15678.2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PTPN14

Protein Tyrosine Phosphatase, Non-receptor type 14 (PTPN14), also called Phosphatase with Ezrin Domain (PEZ) and PTP36, is a 135 kDa cytosolic protein that dephosphorylates tyrosine residues in proteins. PTPN14’s Ezrin domain is homologous to erythrocyte Band 4.1 protein, targeting the phosphatase to cytoskeletal elements such as actin. PTPN14 associates with beta-catenin in adhesion zones in cells that are touching, but is nuclear in isolated, proliferating cells. Loss-of-function mutations in PTPN14 are found in 26% of colorectal cancers, suggesting it is a tumor suppressor.

Long Name

Protein Tyrosine Phosphatase Non-receptor Type 14

Entrez Gene IDs

5784 (Human); 19250 (Mouse); 305064 (Rat)

Alternate Names

EC 3.1.3; EC 3.1.3.48; MGC126803; PEZ; PEZcytoskeletal-associated protein tyrosine phosphatase; protein tyrosine phosphatase, non-receptor type 14; Protein-tyrosine phosphatase pez; PTP36; PTPN14; tyrosine-protein phosphatase non-receptor type 14