Human/Rat PKC beta 1 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
AF3759T-100UG

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Human/Rat PKC beta 1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat PKC beta 1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human PKCδ is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PKCβ1
Lys607-Val671
Accession # P05771
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PKC beta 1

PKC beta 1 is a 671 aa, 77 kDa member of the PKC subfamily of Ser/Thr family of protein kinases. PKC beta 1 is a Ca2+-activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC beta 2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues. In monkey, five alternativly spliced forms have been reported. Over the region used as immunogen, human and rat PKC beta 1 are 100% identical.

Long Name
Protein Kinase C beta 1
Entrez Gene IDs
5579 (Human); 18751 (Mouse); 25023 (Rat)
Alternate Names
PKC beta 1; PKCB;PKCbeta;PKC-beta;PKCI(2);PRKCB;PRKCB1;PRKCB2;Protein kinase C beta type; PRKCB; PRKCB1; PRKCB2

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