PTK7/CCK4 Antibody (525222)

R&D Systems | Catalog # MAB4499

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 525222
View all PTK7/CCK4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308

Specificity

Detects human and rat PTK7/CCK4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PTK7/CCK4 Antibody (525222)

Detection of Human and Rat PTK7/CCK4 antibody by Western Blot.

Detection of Human and Rat PTK7/CCK4 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PTK7/CCK4 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

PTK7/CCK4 in Human Colon Cancer Tissue.

PTK7/CCK4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human PTK7/CCK4 antibody by Simple WesternTM.

Detection of Human PTK7/CCK4 by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 168 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for PTK7/CCK4 Antibody (525222)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Simple Western

20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTK7/CCK4

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, PTK7 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner. PTK7 may also play a role during gastrulation in the rodent embryo.

Long Name

Protein Tyrosine Kinase 7

Alternate Names

CCK4

Entrez Gene IDs

5754 (Human); 71461 (Mouse); 301242 (Rat)

Gene Symbol

PTK7

UniProt

Q13308

Additional PTK7/CCK4 Products

Product Documents for PTK7/CCK4 Antibody (525222)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PTK7/CCK4 Antibody (525222)

For research use only

Citations for PTK7/CCK4 Antibody (525222)

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Protocols

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