Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all S100A16 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A16
Ser2-Ser103
Accession # Q96FQ6

Specificity

Detects human S100A16 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse S100A16 and less than 1% cross-reactivity with recombinant human (rh) S100A13 and rhS100B.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human S100A16 Antibody

S100A16 antibody in Human Brain by Immunohistochemistry (IHC-P).

S100A16 in Human Brain.

S100A16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human S100A16 antibody by Western Blot.

Detection of Human S100A16 by Western Blot.

Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human S100A16 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A16

S100A16 (also S100F) is a 12 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. By Northern blot, S100A16 is found in multiple tissue types; in brain, it is specifically found in astrocytes. Intracellularly, S100A16 is both nuclear and cytoplasmic and apparently exists as a homodimer, trimer and tetramer.

Long Name

S100 Calcium Binding Protein A16

Alternate Names

AAG13, DT1P1A7, S100F

Entrez Gene IDs

140576 (Human); 67860 (Mouse); 361991 (Rat)

Gene Symbol

S100A16

UniProt

Q96FQ6

Additional S100A16 Products

Product Documents for Human S100A16 Antibody

Certificate of Analysis

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Product Specific Notices for Human S100A16 Antibody

For research use only

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Protocols

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