Human Serum Amyloid A4 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3856X Datasheet / COA / SDS

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AF3856X-100UG

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Human Serum Amyloid A4 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serum Amyloid A4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant mouse Serum Amyloid A4 is observed and less than 5% cross-reactivity with recombinant human (rh) Serum

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Serum Amyloid A4
Glu19-Tyr130
Accession # P35542

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Serum Amyloid A4

Serum Amyloid A4 (SAA4) is a constitutively expressed SAA protein that is also known as C-SAA. It is an apolipoprotein that constitutes more than 90% of SAA on HDL during homeostasis, and may be involved in lipid transfer between lipoprotein classes. Mature human SAA4 contains 112 amino acid (aa) residues. It is primarily produced in hepatocytes and secreted plasma. In solution, SAA4 is reported to form stable dimers and trimers. Human and mouse SAA4 share 57% aa sequence identity.

Entrez Gene IDs

6291 (Human); 20211 (Mouse); 365245 (Rat)

Alternate Names

C-SAA; C-SAACSAAConstitutively expressed serum amyloid A protein; SAA4; SAA5; serum amyloid A-4 protein; Serum Amyloid A4; serum amyloid A4, constitutive