Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 706045
View all Siglec-16 Primary Antibodies »

Product Specifications

Immunogen

Human Siglec-16 peptide
Accession # A6NMB1

Specificity

Detects human Siglec-16 in direct ELISAs. In flow cytometry, detects human Siglec-16, but not human Siglec-11, in transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Siglec‑16 Antibody

Detection of Siglec-16 antibody in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP antibody by Flow Cytometry

Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with (A) human Siglec-16 or (B) human Siglec-11, and eGFP was stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68191) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).View our protocol for Staining Membrane-associated Proteins.

Detection of Siglec‑16 in CHO cells transfected with human Siglec-16 or irrelevant transfectant cells by Flow Cytometry.

CHO cells transfected with human Siglec-16 (filled histogram) or irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68191), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Applications for Human Siglec‑16 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP and CHO cells transfected with human Siglec-16 or irrelevant transfectant cells

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: CHO cells transfected with human Siglec-16 or irrelevant transfectant

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Siglec-16

Siglec-16 (Sialic acid-binding Ig-like lectin 16) is a 58-60 kDa member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an alpha 2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM) Lys that interacts with DAP12. Mature human Siglec-16 is a 465 amino acid (aa) type I TM glycoprotein. It contains a 418 aa extracellular region (aa 17-434) that shows one V-type (aa 19-122) plus three C2-type (aa 147-424) Ig‑like domains, and a short 26 aa cytoplasmic tail. Notably, Siglec-16 exists as a pseudogene in approximately 50% of surveyed population. There is no Siglec-16 counterpart in either rodent or distant primate such as Rhesus monkey. The extracellular domain (ECD) of human Siglec-16 shares 94% aa identity with the ECD of human Siglec-11.

Long Name

Sialic Acid Binding Ig-like Lectin 16

Alternate Names

Siglec16, SIGLECP16

Entrez Gene IDs

400709 (Human)

Gene Symbol

SIGLEC16

UniProt

A6NMB1 (Human)

Additional Siglec-16 Products

Product Documents for Human Siglec‑16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Siglec‑16 Antibody

For research use only

Customer Reviews for Human Siglec‑16 Antibody

There are currently no reviews for this product. Be the first to review Human Siglec‑16 Antibody and earn rewards!

Have you used Human Siglec‑16 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies