Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.
Human Siglec-5/Siglec-14 Antibody (194111) [Biotin]
R&D Systems | Catalog # BAM10722
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair)
Cited:
Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 194111
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14
Lys18-Thr434
Accession # O15389
Lys18-Thr434
Accession # O15389
Specificity
Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Siglec-5/Siglec-14 Antibody (194111) [Biotin]
Human Siglec-5 / Siglec-14 ELISA Standard Curve
Recombinant Human Siglec‑5/CD170 Fc Chimera (Catalog # 1072-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑5/Siglec‑14 Monoclonal Antibody (Catalog # MAB10721) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec‑5/Siglec‑14 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Siglec-5/Siglec-14 Antibody (194111) [Biotin]
Human Siglec-5 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Siglec-5/Siglec-14
Long Name
Sialic Acid Binding Ig-like Lectin 5
Alternate Names
CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5
Entrez Gene IDs
8778 (Human)
Gene Symbol
SIGLEC5
UniProt
Additional Siglec-5/Siglec-14 Products
Product Documents for Human Siglec-5/Siglec-14 Antibody (194111) [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Siglec-5/Siglec-14 Antibody (194111) [Biotin]
For research use only
Citations for Human Siglec-5/Siglec-14 Antibody (194111) [Biotin]
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