Human ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Antibody (719508)

R&D Systems | Catalog # MAB67151

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 719508
View all ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SGalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5
Gly30-Phe336
Accession # Q9BVH7

Specificity

Detects human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ST6GALNAC4 or rhST6GALNAC6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images

GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC).

GalNAc alpha ‑2,6‑sialyltransferase V/ST6GALNAC5 in MDA‑MB‑231 Human Cell Line.

GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB67151) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MDA‑MB‑231 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases (2). ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4(NeuAc alpha 2,3)Gal beta 1,4Glc beta 1-Cer) from GM1b (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4Gal beta 1,4Glc beta 1-Cer), and its expression is restricted to the brain (3, 4). ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier (5, 6). The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (7) suggesting the substrate specificity of ST6GALNAC5 may require further characterization.

References

  1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
  2. Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
  3. Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
  4. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
  5. Bos, P.D. et al. (2009) Nature 459:1005.
  6. Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509.
  7. Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.

Long Name

ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 5

Alternate Names

GD1 alpha synthase, SIAT7E, ST6GalNAcV

Entrez Gene IDs

81849 (Human); 26938 (Mouse)

Gene Symbol

ST6GALNAC5

UniProt

Q9BVH7

Additional ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Products

Product Documents

Certificate of Analysis

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Product Specific Notices

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Antibody (719508)

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