Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TBK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBK-1
Met1-Gly121
Accession # Q9UHD2

Specificity

Detects human TBK-1 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TBK1 Antibody

TBK1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

TBK1 in Human Prostate Cancer Tissue.

TBK1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human TBK1 by Simple WesternTM.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for TBK1 at approximately 90 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Applications for Human TBK1 Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Simple Western

20 µg/mL
Sample: Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TBK1

TANK binding kinase-1 (TBK1), encoded by the TBK1 gene, is a multimeric Serine/threonine-protein kinase that mediates a crosstalk between the innate immune response and autophagy. TBK1 was first identified as a TANK-interacting protein controlling NF-kappa B-mediated responses. Mutations in TBK1 have been found in conditions like amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), normal tension glaucoma (NTG) and childhood herpes simplex encephalitis (HSE). Gain-of-function of TBK1 are associated with NTG, whereas loss-of-function mutations result in ALS/FTD or in HSE.

Long Name

TANK Binding Kinase 1

Alternate Names

FTDALS4, NAK, NFKB-Activating Kinase

Entrez Gene IDs

29110 (Human); 56480 (Mouse); 299827 (Rat)

Gene Symbol

TBK1

UniProt

Q9UHD2

Additional TBK1 Products

Product Documents for Human TBK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TBK1 Antibody

For research use only

Customer Reviews for Human TBK1 Antibody

There are currently no reviews for this product. Be the first to review Human TBK1 Antibody and earn rewards!

Have you used Human TBK1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies