Human TNF RII/TNFRSF1B Antibody [Biotin]

R&D Systems | Catalog # BAF726

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, Antibody Array Development, Array Development, ELISA Capture, ELISA Detection, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TNF RII/TNFRSF1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human sTNF RII/TNFRSF1B

Specificity

Detects human TNF RII/TNFRSF1B in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human TNF RI, recombinant mouse (rm) TNF RI, and rmTNF RII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TNF RII/TNFRSF1B Antibody [Biotin]

Human TNF RII / TNFRSF1B ELISA Standard Curve

Human TNF RII / TNFRSF1B ELISA Standard Curve

Recombinant Human TNF RII/TNFRSF1B aa 24-206 (Catalog # 1089-R2) was serially diluted and captured by Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human TNF RII/TNFRSF1B Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)

Human TNF RII/TNFRSF1B Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human TNF RII/TNFRSF1B Antibody (Catalog # MAB726)
  • Standard: Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein (Catalog # 1089-R2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF726 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF RII/TNFRSF1B

TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

Additional TNF RII/TNFRSF1B Products

Product Documents for Human TNF RII/TNFRSF1B Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TNF RII/TNFRSF1B Antibody [Biotin]

For research use only

Citations for Human TNF RII/TNFRSF1B Antibody [Biotin]

Customer Reviews for Human TNF RII/TNFRSF1B Antibody [Biotin] (1)

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  • Human TNF RII/TNFRSF1B Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma and Cell culture supernatant
    Species: Human
    Verified Customer | Posted 11/22/2017
    We used MAB726 as the solid phase antibody, 1089-R2 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.
    Human TNF RII/TNFRSF1B Antibody [Biotin] BAF726

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