Human TRAIL R3/TNFRSF10C Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB6301V Datasheet / COA / SDS

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FAB6301V-100UG

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Human TRAIL R3/TNFRSF10C Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAIL R3/TNFRSF10C in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, or rhTRAIL R4 is observed.

Source

Monoclonal Mouse IgG1 Clone # 90905

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C
Ala26-Ala221
Accession # O14798

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TRAIL R3/TNFRSF10C

Human TRAIL R3, also called DcR1 (decoy receptor 1), LIT, and TRID and designated TNFRSF10C, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

Long Name

TRAIL Receptor 3

Entrez Gene IDs

8794 (Human)

Alternate Names

Antagonist decoy receptor for TRAIL/Apo-2L; CD263 antigen; CD263; cytotoxic TRAIL receptor-3; DcR1; DCR1MGC149502; Decoy receptor 1; Decoy TRAIL receptor without death domain; LITMGC149501; Lymphocyte inhibitor of TRAIL; TNFRSF10C; TRAIL R3; TRAIL receptor 3; TRAIL receptor without an intracellular domain; TRAILR3; TRAIL-R3; TRAILR3TNF-related apoptosis-inducing ligand receptor 3; TRIDDCR1-TNFR; tumor necrosis factor receptor superfamily member 10C; tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain