Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 415909
View all TFF3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654

Specificity

Detects human TFF3 in direct ELISAs and Western blots. Does not cross-react with recombinant human TFF2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Trefoil Factor 3 Antibody

TFF3 antibody in Human Colon by Immunohistochemistry (IHC-P).

TFF3 in Human Colon.

TFF3 was detected in immersion fixed paraffin-embedded sections of human colon using 25 µg/mL Human TFF3 Monoclonal Antibody (Catalog # MAB4407) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of goblet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Trefoil Factor 3 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

1 µg/mL
Sample: Recombinant Human TFF3
under non-reducing conditions only

Reviewed Applications

Read 5 reviews rated 4.4 using MAB4407 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TFF3

Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 aa, 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Entrez Gene IDs

7033 (Human); 21786 (Mouse); 25563 (Rat)

Gene Symbol

TFF3

UniProt

Q07654

Additional TFF3 Products

Product Documents for Human Trefoil Factor 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Trefoil Factor 3 Antibody

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Human Trefoil Factor 3 Antibody

Customer Reviews for Human Trefoil Factor 3 Antibody (5)

4.4 out of 5
5 Customer Ratings
5 Stars
40%
4 Stars
60%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 5 reviews Showing All
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  • Human Trefoil Factor 3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 10/27/2021
    Human Trefoil Factor 3 Antibody MAB4407
  • Human Trefoil Factor 3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human Trefoil Factor 3 Antibody MAB4407
  • Human Trefoil Factor 3 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Trefoil Factor 3 Antibody MAB4407
  • Human Trefoil Factor 3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018
  • Human Trefoil Factor 3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018

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Protocols

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