Human TTF-1/NKX2-1 Antibody (2054E)

R&D Systems | Catalog # MAB94581

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2054E
View all TTF-1/NKX2-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TTF-1/NKX2-1
Ala40-Pro111
Accession # P43699

Specificity

Detects human TTF-1/NKX2-1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human TTF-1/NKX2-1 Antibody (2054E)

Detection of Human TTF-1/NKX2-1 antibody by Western Blot.

Detection of Human TTF-1/NKX2-1 by Western Blot.

Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB94581) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TTF-1/NKX2-1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TTF-1/NKX2-1 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

TTF-1/NKX2-1 in A549 Human Cell Line.

TTF-1/NKX2-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB94581) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TTF-1/NKX2-1 antibody in Human Thyroid by Immunohistochemistry (IHC-P).

TTF-1/NKX2-1 in Human Thyroid.

TTF-1/NKX2-1 was detected in immersion fixed paraffin-embedded sections of human thyroid using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB94581) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of TTF-1/NKX2-1 by Immunocytochemistry/ Immunofluorescence

Detection of TTF-1/NKX2-1 by Immunocytochemistry/ Immunofluorescence

AT2 generation and characterization. (a) A schematic outline of the AT2 differentiation protocol. (b) Morphological and ICC analysis of the generated AT2s’ 3D alveolospheres showing expression of AT2-specific markers NKX2-1, EpCAM, SFTPB, and SFTPC. (c) (i,ii) Representative enlarged images detailing HCS quantification methodology of the nuclear and cytoplasmic expression of AT2 markers: (i) quantification of NKX2-1 nuclear expression, (ii) expression of EpCAM, SFTPB, and SFTPC markers measured in the cytoplasmic area marked by the white line minus the nuclear area. (d) Representative ICC images of each AT2 marker expression in monolayer culture; expression of each marker was quantitatively measured, as detailed in ‘c’, and cells passing a fluorescence threshold, set uniformly across all samples, were counted positive for the respective marker and identified as red/green highlighted nuclei. (e) Bar graphs showing the percentage of cells positive for each AT2 marker across 10 generated AT2 lines. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2073-4409/13/5/369), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TTF-1/NKX2-1 Antibody (2054E)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample:

Immersion fixed A549 human lung carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thyroid

Western Blot

1 µg/mL
Sample: TT human medullary thyroid cancer cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB94581 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TTF-1/NKX2-1

NK2 homeobox 1 (NKX2-1), also known as thyroid transcription factor 1 (TTF-1), is a 371 aminoacids (aa) protein encoded by the NKX2-1 gene. Expression of NKX2-1 transcription factor is the earliest indication of the establishment of respiratory progenitors as well as thyroid epithelium in the ventral foregut endoderm. NKX2-1 is critical for the expression of many pulmonary specific genes, including surfactant proteins SP-A, -B, and –C, as well as thyroid specific genes that are important in the maintenance of the thyroid differentiation phenotype.

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Entrez Gene IDs

7080 (Human); 21869 (Mouse); 25628 (Rat)

Gene Symbol

NKX2-1

UniProt

P43699

Additional TTF-1/NKX2-1 Products

Product Documents for Human TTF-1/NKX2-1 Antibody (2054E)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TTF-1/NKX2-1 Antibody (2054E)

For research use only

Citations for Human TTF-1/NKX2-1 Antibody (2054E)

Customer Reviews for Human TTF-1/NKX2-1 Antibody (2054E) (2)

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Customer Images

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  • Human TTF-1/NKX2-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Squamous cell carcinoma
    Species: Human
    Verified Customer | Posted 11/08/2021
    Human TTF-1/NKX2-1 Antibody (2054E) MAB94581
  • Human TTF-1/NKX2-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: BRONCHUS
    Species: Human
    Verified Customer | Posted 02/15/2019
    Human TTF-1/NKX2-1 Antibody (2054E) MAB94581

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