Human VAMP-8 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF5354X Datasheet / COA / SDS

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AF5354X-100UG

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Human VAMP-8 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VAMP-8 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human VAMP-8
Met1-Lys75
Accession # Q9BV40

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: VAMP-8

VAMP-8 (vesicle associated membrane protein 8; also endobrevin) is a 12 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is predominately transmembrane) that is found in cells involved in vesicle release (platelets, mast cells and pancreatic acinar cells). It regulates exocytosis and decouples granule release from cytokine release. Human VAMP-8 is 100 amino acids (aa) in length. It contains a vSNARE coiled-coil homology region (aa 12-72) and a membrane-anchor domain (aa 76‑96). Over aa 1‑75, human VAMP-8 shares 93% aa identity with mouse
VAMP‑2.

Long Name

Vesicle-Associated Membrane Protein 8

Entrez Gene IDs

8673 (Human); 22320 (Mouse); 83730 (Rat)

Alternate Names

EDB; EDBVAMP-8; Endobrevin; VAMP8; VAMP-8; vesicle-associated membrane protein 8 (endobrevin); vesicle-associated membrane protein 8