Human Wnt‑7b Antibody

Detection of Wnt‑7b in Human Breast Cancer

Wnt‑7b was detected in immersion fixed paraffin-embedded sections of human breast cancer using Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Wnt-7b by Immunocytochemistry/ Immunofluorescence

WNT7b was overexpressed in colorectal cells. a Representative images of immunofluorescence staining of WNT7b subcellular localization of FHC and HCT116 cells. Nuclei were counterstained with DAPI. Scale bar equals to 20 μm. b and c Cytoplasmic, nuclear, and membrane lysate of FHC and HCT116 cells were separately extracted and analyzed by Western Blot, with GAPDH, Lamin B1, and Na/K ATPase alpha 1 as the loading control, respectively. Quantification of the WNT7b expression was analyzed by image J, P value was shown and calculated by student’s t test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33607955), licensed under a CC-BY license. Not internally tested by R&D Systems.