IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-12035
![Flow Cytometry: IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free [NBP3-12035]](https://resources.rndsystems.com/images/products/IL-17-IL-17A-Antibody-MM17F3-Chimeric-Flow-Cytometry-NBP3-12035-img0001.jpg "Flow Cytometry: IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free [NBP3-12035]")
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Scientific Data Images for IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free
Flow Cytometry: IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free [NBP3-12035]
Flow Cytometry: IL-17/IL-17A Antibody (MM17F3) - Chimeric [NBP3-12035] - Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (NBP3-12035, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.Applications for IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free
Block/Neutralize
ELISA
Flow Cytometry
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
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Background: IL-17/IL-17A
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Additional IL-17/IL-17A Products
Product Documents for IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for IL-17/IL-17A Antibody (MM17F3) - Chimeric - Azide and BSA Free
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Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
A:
Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
A:
Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
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Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
A:
Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
A:
Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
Th17 Differentiation Pathway
