Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all IL-27 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human IL-27. The immunogen is located within the first 50 amino acids of IL-27. Amino Acid Squence: WGFPRPPGRPQLSLQE

Reactivity Notes

0

Specificity

Human IL-27 has one isoform (243aa, 28kD). Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD). NBP1-76698 can detect human, mouse and rat IL-27.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for IL-27 Antibody [HRP]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-27

IL27 is encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Long Name

Interleukin 27

Alternate Names

IL27

Gene Symbol

IL27

Additional IL-27 Products

Product Documents for IL-27 Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-27 Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Th1 Differentiation Pathway Th1 Differentiation Pathway Thumbnail