Key Product Details

Validated by

Independent Antibodies, Tagged Protein Expression

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IL-27 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human IL-27. The immunogen is located within the first 50 amino acids of IL-27. Amino Acid Squence: WGFPRPPGRPQLSLQE

Specificity

Human IL-27 has one isoform (243aa, 28kD). Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD). NBP1-76698 can detect human, mouse and rat IL-27.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit IL-27 Antibody - BSA Free (NBP1-76698) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IL-27 Antibody - BSA Free

Western Blot: IL-27 AntibodyBSA Free [NBP1-76698]

Western Blot: IL-27 AntibodyBSA Free [NBP1-76698]

Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.
Western Blot: IL-27 AntibodyBSA Free [NBP1-76698]

Western Blot: IL-27 AntibodyBSA Free [NBP1-76698]

Western Blot: IL-27 Antibody [NBP1-76698] - Analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: IL-27 AntibodyBSA Free [NBP1-76698]

Western Blot: IL-27 AntibodyBSA Free [NBP1-76698]

Western Blot: IL-27 Antibody [NBP1-76698] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: IL-27 NBP1-76698 (4 ug/mL), IL-27 NBP1-76412 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IL-27 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IL-27 Antibody - BSA Free [NBP1-76698] -

Immunocytochemistry/ Immunofluorescence: IL-27 Antibody - BSA Free [NBP1-76698] - Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for IL-27 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

0.5-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-27

Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.

Long Name

Interleukin 27

Alternate Names

IL27

Gene Symbol

IL27

UniProt

NP_663634

Additional IL-27 Products

Product Documents for IL-27 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IL-27 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-27 Antibody - BSA Free

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    • A: We recommend using any Rabbit IgG secondary. You can use any conjugate you want depending on your detection system. Most commonly we use HRP. Use the conjugate filter on the left to sort for the one that will be suitable for you.
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Associated Pathways

Th1 Differentiation Pathway Th1 Differentiation Pathway Thumbnail