Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13709
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Western Blot: Influenza B nucleoprotein Antibody (HL1069)(B/Taiwan/753/2005) [NBP3-13709]
Western Blot: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Influenza B viral lysates (1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
Immunocytochemistry/Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Immunofluorescent analysis of influenza virus infected cells using Influenza B Nucleoprotein antibody (NBP3-13709). Sample: Influenza A and B infected cells slide. Green: Influenza B Nucleoprotein antibody (NBP3-13709) diluted at 1:100.Immunocytochemistry/ Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
Immunocytochemistry/Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Influenza B Nucleoprotein antibody (NBP3-13709) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza B Nucleoprotein stained by Influenza B Nucleoprotein antibody (NBP3-13709) diluted at 1:2000.Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Detection of recombinant influenza B nucleoprotein(B/Sydney/3/2004), DDDDK Tag by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1068 : Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708) HL1069 : Influenza B Virus Nucleoprotein antibody [HL1069] (NBP3-13709) HL1073 : Influenza B Nucleoprotein antibody [HL1073] (NBP3-13713) Sample : Influenza B Virus Nucleoprotein (B/Sydney/3/2004), DDDDK TagELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A (i.e., A/California/07/09 (H1N1)) and Influenza B (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 ug). Coated protein was probed with Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (1 ug/mL). Rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis was performed by coating a plate with viral lysates (1 ug) derived from different strains of influenza B or influenza A and probing with the specified influenza B nucleoprotein antibodies (1 ug/ml). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) or goat anti-mouse IgG antibody (HRP) (NBP2-19382) (1:10000) were used to detect the bound primary antibodies.Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Detection of recombinant influenza B nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) Detection: Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708)ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis was performed by coating a plate with viral lysates (1 ug) derived from different strains of influenza B or influenza A and probing with the specified influenza B nucleoprotein antibodies (1 ug/ml). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) or goat anti-mouse IgG antibody (HRP) (NBP2-19382) (1:10000) were used to detect the bound primary antibodies.ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B (nucleoprotein) protein, DDDDK tag (50 ng). Coated protein was probed with Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (20000-4.77*10^-3 pM). Rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody. EC50 : 8.72 pMELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]
ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis was performed by coating a plate with recombinant influenza B nucleoprotein (B/Sydney/3/2004), DDDDK Tag (50 ng), and probing with the specified influenza B nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) or goat anti-mouse IgG antibody (HRP) (NBP2-19382) (1:10000) were used to detect the bound primary antibodies.Western Blot: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709] -
Influenza B viral lysate was separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709] -
Influenza A viral lysate and Influenza B viral lysate (0.1 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications
ELISA
Immunocytochemistry/ Immunofluorescence
Lateral Flow Assay
Sandwich ELISA
Western Blot
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Background: Influenza B nucleoprotein
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Additional Influenza B nucleoprotein Products
Product Documents
Certificate of Analysis
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Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs
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Q: We ordered your Influenza A nucleoprotein. We tested it. And it worked well for us. I am wondering if you also have Influenza B nucleoprotein, Recombinant. I don’t see it in your catalog.
A: At this time, it appears we only carry antibodies direct towards Influenza B nucleoprotein. We do not currently have a recombinant protein to this target. I apologize for the inconvenience.