Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13709

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Virus

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Lateral Flow Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1069 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza B nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Reactivity Notes

Influenza B virus

Specificity

Influenza B Nucleoprotein (B/Taiwan/753/2005)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images

Western Blot: Influenza B nucleoprotein Antibody (HL1069)(B/Taiwan/753/2005) [NBP3-13709]

Western Blot: Influenza B nucleoprotein Antibody (HL1069)(B/Taiwan/753/2005) [NBP3-13709]

Western Blot: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Influenza B viral lysates (1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Immunocytochemistry/ Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Immunocytochemistry/Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Immunofluorescent analysis of influenza virus infected cells using Influenza B Nucleoprotein antibody (NBP3-13709). Sample: Influenza A and B infected cells slide. Green: Influenza B Nucleoprotein antibody (NBP3-13709) diluted at 1:100.
Immunocytochemistry/ Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Immunocytochemistry/ Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Immunocytochemistry/Immunofluorescence: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Influenza B Nucleoprotein antibody (NBP3-13709) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza B Nucleoprotein stained by Influenza B Nucleoprotein antibody (NBP3-13709) diluted at 1:2000.
Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Detection of recombinant influenza B nucleoprotein(B/Sydney/3/2004), DDDDK Tag by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1068 : Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708) HL1069 : Influenza B Virus Nucleoprotein antibody [HL1069] (NBP3-13709) HL1073 : Influenza B Nucleoprotein antibody [HL1073] (NBP3-13713) Sample : Influenza B Virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of recombinant Influenza B Nucleoprotein (B/Sydney/3/2004), DDDDK Tag using antibodies as below. Capture: Influenza B Virus Nucleoprotein antibody [HL1069] (NBP3-13709) (5 ug/mL) Detection: HRP-conjugated Influenza B Nucleoprotein antibody [HL1073] (NBP3-13713) (1 ug/mL) Please notice that NBP3-13713 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13709. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of recombinant Influenza B Nucleoprotein (B/Sydney/3/2004), DDDDK Tag using antibodies as below. Capture: Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (5 ug/mL) Detection: HRP-conjugated Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708) (1 ug/mL) Please notice that NBP3-13708 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13709. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below. Capture: Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (5 ug/mL) Detection: HRP-conjugated Influenza B Nucleoprotein antibody [HL1073] (NBP3-13713) (1 ug/mL) Please notice that NBP3-13713 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13709. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below. Capture: Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (5 ug/mL) Detection: HRP-conjugated Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708) (1 ug/mL) Please notice that NBP3-13708 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13709. Please contact us for custom HRP-conjugated antibody.
ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A (i.e., A/California/07/09 (H1N1)) and Influenza B (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 ug). Coated protein was probed with Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (1 ug/mL). Rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.
ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis was performed by coating a plate with viral lysates (1 ug) derived from different strains of influenza B or influenza A and probing with the specified influenza B nucleoprotein antibodies (1 ug/ml). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) or goat anti-mouse IgG antibody (HRP) (NBP2-19382) (1:10000) were used to detect the bound primary antibodies.
Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

Lateral Flow Assay: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Detection of recombinant influenza B nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair. Capture: Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) Detection: Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708)
ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis was performed by coating a plate with viral lysates (1 ug) derived from different strains of influenza B or influenza A and probing with the specified influenza B nucleoprotein antibodies (1 ug/ml). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) or goat anti-mouse IgG antibody (HRP) (NBP2-19382) (1:10000) were used to detect the bound primary antibodies.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708) as capture antibody at concentration of 5 ug/mL and HRP-conjugated Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13709 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13708. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B Nucleoprotein antibody [HL1073] (NBP3-13713) as capture antibody at concentration of 5 ug/mL and HRP-conjugated Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13709 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13713. Please contact us for custom HRP-conjugated antibody.
ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B (nucleoprotein) protein, DDDDK tag (50 ng). Coated protein was probed with Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (20000-4.77*10^-3 pM). Rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody. EC50 : 8.72 pM
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of recombinant nucleoproteins derived from different strains of Influenza B (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below. Capture : Influenza B Nucleoprotein antibody [HL1068] (NBP3-13708) (5 ug/mL) Detection: HRP-conjugated Influenza B Virus Nucleoprotein antibody [HL1069] (NBP3-13709) (1 ug/mL). Please notice that NBP3-13709 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13708. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of recombinant nucleoproteins derived from different strains of Influenza B (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below. Capture: Influenza B Nucleoprotein antibody [HL1073] (NBP3-13713) (5 ug/mL) Detection: HRP-conjugated Influenza B Virus Nucleoprotein antibody [HL1069] (NBP3-13709) (1 ug/mL). Please notice that NBP3-13709 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13713. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of viral lysate derived from different strains of Influenza A (i.e., A/California/07/09 (H1N1)) and Influenza B (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below. Capture: Influenza B Virus Nucleoprotein antibody [HL1068] (NBP3-13708) (5 ug/mL) Detection: HRP-conjugated Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (1 ug/mL) Please notice that NBP3-13709 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13708. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Sandwich ELISA detection of viral lysate derived from different strains of Influenza A (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below. Capture: Influenza B Nucleoprotein antibody [HL1073] (NBP3-13713) (5 ug/mL) Detection: HRP-conjugated Influenza B Nucleoprotein antibody [HL1069] (NBP3-13709) (1 ug/mL) Please notice that NBP3-13709 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13713. Please contact us for custom HRP-conjugated antibody.
ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709]

ELISA: Influenza B nucleoprotein Antibody (HL1069) [NBP3-13709] - Indirect ELISA analysis was performed by coating a plate with recombinant influenza B nucleoprotein (B/Sydney/3/2004), DDDDK Tag (50 ng), and probing with the specified influenza B nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) or goat anti-mouse IgG antibody (HRP) (NBP2-19382) (1:10000) were used to detect the bound primary antibodies.
Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) - Azide and BSA Free

Western Blot: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) - Azide and BSA Free

Western Blot: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709] -

Influenza B viral lysate was separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) - Azide and BSA Free

Western Blot: Influenza B nucleoprotein Antibody (HL1069) - (B/Taiwan/753/2005) [NBP3-13709] -

Influenza A viral lysate and Influenza B viral lysate (0.1 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (NBP3-13709) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

Assay dependent

Lateral Flow Assay

Assay dependent

Sandwich ELISA

Assay dependent

Western Blot

Assay dependent

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza B nucleoprotein

Influenza virus B is a genus in the virus family Orthomyxoviridae. A limited host range means that Influenza virus B pandemics are rare. The nucleoprotein protects the negative strand viral RNA from nucleases by encapsidating it. encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication.

Alternate Names

Influenza B Virus NP, NP, Nucleoprotein

Additional Influenza B nucleoprotein Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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  • Q: We ordered your Influenza A nucleoprotein. We tested it. And it worked well for us. I am wondering if you also have Influenza B nucleoprotein, Recombinant. I don’t see it in your catalog.

    A: At this time, it appears we only carry antibodies direct towards Influenza B nucleoprotein. We do not currently have a recombinant protein to this target. I apologize for the inconvenience.

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