INSM1 Antibody (MSVA-456R)

Novus Biologicals | Catalog # NBP3-57221

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-456R
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Product Specifications

Immunogen

Recombinant fragment (around aa81-125) corresponding to the N-terminus of human INSM1 Swissprot Q01101

Localization

Nuclear

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Pancreas: Islet cells should show a moderate to strong INSM1 immunostaining.

Negative Control
Negitve control:Pancreas: All acinar cells should be negative

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Applications for INSM1 Antibody (MSVA-456R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:75 1:150
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: INSM1

Insulinoma-associated 1 (INSM1) gene is intronless and encodes a protein containing both a zinc finger DNA-binding domain and a putative prohormone domain. This gene is a sensitive marker for neuroendocrine differentiation of human lung tumors. (provided by RefSeq)

Alternate Names

IA1IA-1, insulinoma-associated 1, insulinoma-associated protein 1, Zinc finger protein IA-1

Gene Symbol

INSM1

Additional INSM1 Products

Product Documents for INSM1 Antibody (MSVA-456R)

Certificate of Analysis

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Product Specific Notices for INSM1 Antibody (MSVA-456R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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