Integrin alpha 7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86118

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Mouse

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Integrin alpha 7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FACPLSLEETDCYRVDIDQGADMQKESKENQWLGVSVRSQGPGGKIVTCAHRYEARQRVDQILETRDMIGRCFVLSQDLAIRDELDGGEWKFCE

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33686208). Bovine reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Integrin alpha 7 Antibody - BSA Free (NBP1-86118) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-Integrin alpha 7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin alpha 7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin alpha 7 Antibody [NBP1-86118]

Immunocytochemistry/ Immunofluorescence: Integrin alpha 7 Antibody [NBP1-86118]

Immunocytochemistry/Immunofluorescence: Integrin alpha 7 Antibody [NBP1-86118] - Blue (Dapi), Red (Integrin alpha 7), Green (skeletal muscle Myosin). Mononucleated cells containing satellite cells and fibroblasts were isolated from bovine skeletal muscle tissue and induced for myogenic differentiation. Cells were fixed in methanol and stained using anti-myosin antibody and anti-Integrin a7 (#NBP1-86118) antibody. Corresponding secondary antibodies were used to visualize the result. Strong integrin a7 signal was observed in myotubes. But some weaker signals were also observed in fibroblasts which are known to be negative for it. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Integrin alpha 7 Antibody [NBP1-86118]

Immunohistochemistry-Paraffin: Integrin alpha 7 Antibody [NBP1-86118]

Immunohistochemistry-Paraffin: Integrin alpha 7 Antibody [NBP1-86118] - Staining of human heart muscle shows cytoplasmic and membranous positivity in myocytes.
Immunocytochemistry/ Immunofluorescence: Integrin alpha 7 Antibody [NBP1-86118]

Immunocytochemistry/ Immunofluorescence: Integrin alpha 7 Antibody [NBP1-86118]

Immunocytochemistry/Immunofluorescence: Integrin alpha 7 Antibody [NBP1-86118] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.

Applications for Integrin alpha 7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-86118 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 7

ITGA7 encodes integrin alpha chain 7. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 7 undergoes post-translational cleavage within the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that binds to the extracellular matrix protein laminin-1. Alpha 7 beta 1 is the major integrin complex expressed in differentiated muscle cells. Splice variants of alpha 7 that differ in both the extracellular and cytoplasmic domains exist in the mouse; however, to date only a single human transcript type has been isolated: it contains extracellular and cytoplasmic domains corresponding to the mouse X2 and B variants, respectively. A unique extracellular splice variant has been identified in human, although it clearly represents a minor species and its biological significance is unclear.

Alternate Names

ITGA7

Gene Symbol

ITGA7

Additional Integrin alpha 7 Products

Product Documents for Integrin alpha 7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin alpha 7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin alpha 7 Antibody - BSA Free

Customer Reviews for Integrin alpha 7 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Integrin alpha 7 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: skeletal muscle derived cells
    Species: Bovine
    Verified Customer | Posted 03/20/2018
    Blue (Dapi), Red (Integrin alpha 7), Green (skeletal muscle Myosin)
    Mononucleated cells containing satellite cells and fibroblasts were isolated from bovine skeletal muscle tissue and induced for myogenic differentiation. Cells were fixed in methanol and stained using anti-myosin antibody and anti-Integrin a7 (#NBP1-86118) antibody. Corresponding secondary antibodies were used to visualize the result. Strong integrin a7 signal was observed in myotubes. But some weaker signals were also observed in fibroblasts which are known to be negative for it.
    Integrin alpha 7 Antibody - BSA Free NBP1-86118

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