JAM-C Antibody - BSA Free

Name: JAM-C Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-92032
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-92032

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RDSALYRCEVVARNDRKEIDEIVIELTVQVKPVTPVCRVPKAVPVGKMATLHCQESEGHPRPHYSWYRNDVPLPTDSRANPRFRNSSFHLNSETGTLVFTAVHKDDSGQYYCIASNDAGSARCEEQEMEVYDLNIG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit JAM-C Antibody - BSA Free (NBP1-92032) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-JAM-C Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for JAM-C Antibody - BSA Free

Western Blot: JAM-C Antibody [NBP1-92032]

Western Blot: JAM-C Antibody [NBP1-92032] - Analysis in human cell line BEWO.

Immunocytochemistry/ Immunofluorescence: JAM-C Antibody [NBP1-92032]

Immunocytochemistry/Immunofluorescence: JAM-C Antibody [NBP1-92032] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032]

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032]

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032]

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032]

Immunohistochemistry-Paraffin: JAM-C Antibody [NBP1-92032] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Western Blot: JAM-C Antibody - BSA Free [NBP1-92032]

Analysis in human cell line BEWO.

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Applications for JAM-C Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/mL

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JAM-C

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. [provided by RefSeq]

Long Name

Junctional Adhesion Molecule C

Alternate Names

CD323, JAM3, JAMC

Gene Symbol

JAM3

Additional JAM-C Products

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Product Specific Notices for JAM-C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.