KCC2/SLC12A5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13161

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KCC2/SLC12A5 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse KCC2/SLC12A5. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for KCC2/SLC12A5 Antibody - BSA Free

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161]

Immunocytochemistry/ Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161]

Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161]

Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161]

Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500.
Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
KCC2/SLC12A5 Antibody

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] -

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KCC2/SLC12A5 Antibody

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] -

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KCC2/SLC12A5 Antibody

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] -

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KCC2/SLC12A5 Antibody

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] -

Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Human brain (5 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for KCC2/SLC12A5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KCC2/SLC12A5

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. (provided by RefSeq)

Long Name

K-Cl Cotransporter 2

Alternate Names

SLC12A5

Gene Symbol

SLC12A5

Additional KCC2/SLC12A5 Products

Product Documents for KCC2/SLC12A5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KCC2/SLC12A5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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