KCNJ10 Antibody

Novus Biologicals | Catalog # NBP1-70371

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all KCNJ10 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 330-379 of human KCNJ10 conjugated to blue carrier protein was used as the antigen.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This peptide is soluble in PBS and water.

Scientific Data Images for KCNJ10 Antibody

Western Blot: KCNJ10 Antibody [NBP1-70371]

Western Blot: KCNJ10 Antibody [NBP1-70371]

Western Blot: KCNJ10 Antibody [NBP1-70371] - WB on rat spinal cord lysate. Primary antibody was used at 1:500 dilution incubated overnight at 4C.

Applications for KCNJ10 Antibody

Application
Recommended Usage

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Paraffin

1:300-1:2000

Western Blot

1:300-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KCNJ10

This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes.

Long Name

ATP-sensitive inward rectifier potassium channel 10

Alternate Names

ATP-dependent inwardly rectifying potassium channel Kir4.1, ATP-sensitive inward rectifier potassium channel 10, BIRK-10, glial ATP-dependent inwardly rectifying potassium channel KIR4.1, Inward rectifier K(+) channel Kir1.2, inward rectifier K+ channel KIR1.2, KCNJ13-PEN, Kir1.2, KIR4.1, Potassium channel, inwardly rectifying subfamily J member 10, potassium inwardly-rectifying channel, subfamily J, member 10, SESAME

Gene Symbol

KCNJ10

Additional KCNJ10 Products

Product Documents for KCNJ10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KCNJ10 Antibody

This peptide is soluble in PBS and water.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KCNJ10 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for KCNJ10 Antibody

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  • Q: Which specific peptide sequence are detected by this antibody? It is only stated in the data sheet that it reacts against a part of the C-terminal part of KIR4.1. Also, do you know if this antibody, or any of your other antibodies, could be utilized as an intracellular staining of cells for flow cytometry? Furthermore, I have seen in the data sheet for the synthetic peptide that it is not suitable for use with live cells, but is this an absolute? If this is the case, would it possible to buy a similar peptide which could be used with live cells, and if so at which prize/amount? Lastly, are the peptide in a frozen liquid form (or solid powder) with out trace of azide? When it comes to the cell lysate I'm wondering if this could be utilized with live cells or if the RIPA buffer which it is dissolved in makes this very toxic? If the last is the case, is it possible to provide the lysate in another buffer which are appropriate for live cells? Lastly, I wondered if the transfected HEK293T cells are also sold for culture?

    A:

    I can disclose that the immunogen sequence of this antibody corresponds to a region within amino acids 330-379 of human KCNJ10. This antibody has not yet been tested in flow cytometry (you can see this as flow is not listed as a tested application in the product information), so we cannot guarantee that it would be suitable for this application. However, if you would be interested in testing this novel application, please take a look at our Innovator's Reward program. I have searched for an alternative product for you, but unfortunately none of our KCNJ10 antibodies currently have flow cytometry as a validated application.

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