KDELR1 Antibody (KR-10) - BSA Free

Novus Biologicals | Catalog # NBP2-12873

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Hamster, Mammal, Monkey, Rabbit, Sheep, Xenopus

Cited:

Mammal

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # KR-10

Format

BSA Free
View all KDELR1 Primary Antibodies »

Product Specifications

Immunogen

A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH

Reactivity Notes

Use in Mammal reported in scientific literature (PMID:31653700).

Localization

Endoplasmic Reticulum

Specificity

Detects approx 25kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KDELR1 Antibody (KR-10) - BSA Free

Western Blot: KDELR1 Antibody (KR-10) [NBP2-12873]

Western Blot: KDELR1 Antibody (KR-10) [NBP2-12873]

Western Blot: KDELR1 Antibody (KR-10) [NBP2-12873] - analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: KDELR1 Antibody (KR-10) [NBP2-12873]

Immunocytochemistry/ Immunofluorescence: KDELR1 Antibody (KR-10) [NBP2-12873]

Immunocytochemistry/Immunofluorescence: KDELR1 Antibody (KR-10) [NBP2-12873] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDELR1 Monoclonal Antibody, Clone KR-10 (NBP2-12873). Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDELR1 Monoclonal Antibody (NBP2-12873) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.

Applications for KDELR1 Antibody (KR-10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/ml was sufficient for detection of KDEL receptor in 20 ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Reviewed Applications

Read 1 review rated 4 using NBP2-12873 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KDELR1

1ug/ml was sufficient for detection of KDEL receptor in 20ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG: AP as the secondary.

Alternate Names

ER lumen protein retaining receptor 1, ERD2, ERD2.1KDEL receptor 1, HDEL, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, PM23, Putative MAPK-activating protein PM23

Gene Symbol

KDELR1

UniProt

Q99JH8 (Mouse)

Additional KDELR1 Products

Product Documents for KDELR1 Antibody (KR-10) - BSA Free

Certificate of Analysis

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Product Specific Notices for KDELR1 Antibody (KR-10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KDELR1 Antibody (KR-10) - BSA Free

Customer Reviews for KDELR1 Antibody (KR-10) - BSA Free (1)

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  • KDELR1 Antibody (KR-10)
    Name: Boyan Zhang
    Application: Immunocytochemistry
    Sample Tested: RPE1 cells
    Species: Human
    Verified Customer | Posted 09/07/2021
    RPE1 cells were methanol fixed, co-stained with KDELR1 (red) and Giantin (green).
    KDELR1 Antibody (KR-10) - BSA Free NBP2-12873

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