KIF22 Antibody

Novus Biologicals | Catalog # NBP2-17053

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (92%), Chimpanzee (99%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all KIF22 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human KIF22. The exact sequence is proprietary.

Reactivity Notes

Mouse (88%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KIF22 Antibody

Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053]

Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053]

Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053]

Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053]

Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053] - Analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
KIF22 Antibody

Western Blot: KIF22 Antibody [NBP2-17053] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF22 antibody [N3C2], Internal (NBP2-17053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KIF22 Antibody

Immunocytochemistry/ Immunofluorescence: KIF22 Antibody [NBP2-17053] -

KIF22 antibody [N3C2], Internal detects KIF22 protein at cytoskeleton by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KIF22 protein stained by KIF22 antibody [N3C2], Internal (NBP2-17053) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Applications for KIF22 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: KIF22

The protein encoded by this gene is a member of kinesin-like protein family. This family of proteins are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. [provided by RefSeq]

Alternate Names

Kid, KIDA-328A3.2, kinesin family member 22, kinesin-like 4, Kinesin-like DNA-binding protein, Kinesin-like protein 4, kinesin-like protein KIF22, KNSL4kinesin-like DNA-binding protein pseudogene, OBP, OBP-1, OBP-2, origin of plasmid DNA replication-binding protein, oriP binding protein

Gene Symbol

KIF22

UniProt

Q14807 (Human)

Additional KIF22 Products

Product Documents for KIF22 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KIF22 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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