Kif4A Antibody (4A9P2)

Novus Biologicals | Catalog # NBP3-16748

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4A9P2 expressed in HEK293
View all Kif4A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KIF4A (O95239). MKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kif4A Antibody (4A9P2)

Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748]

Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748]

Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748]

Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748]

Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded mouse testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748]

Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748]

Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded rat testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748]

Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748]

Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded human colon using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Kif4A Antibody (4A9P2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kif4A

The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF14 silencing experiments suggest that KIF14 plays a multi-functional role in mitosis and cytokinesis. KIF4A has been shown to be essential for cytokinesis. KIF4A is also known as kinesin family member 4A, chromosome-associated kinesin KIF4A, chromokinesin, KIF4, KIF4-G1, FLJ12530, FLJ12655, FLJ14204, FLJ20631, and HSA271784.

Alternate Names

chromokinesin-A, chromosome-associated kinesin KIF4A, FLJ12530, FLJ12655, FLJ14204, FLJ20631, HSA271784, KIF4G1, KIF4KIF4-G1, kinesin family member 4A

Gene Symbol

KIF4A

Additional Kif4A Products

Product Documents for Kif4A Antibody (4A9P2)

Certificate of Analysis

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Product Specific Notices for Kif4A Antibody (4A9P2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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