LDB3 Antibody

Novus Biologicals | Catalog # NBP2-19343

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all LDB3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDB3. The exact sequence is proprietary.

Reactivity Notes

Chicken (84%).

Localization

Cytoplasm; perinuclear region; Cell projection; pseudopodium; cytoskeleton; myofibril; sarcomere; Z-disk

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LDB3 Antibody

Immunohistochemistry-Paraffin: LDB3 Antibody [NBP2-19343]

Immunohistochemistry-Paraffin: LDB3 Antibody [NBP2-19343]

Immunohistochemistry-Paraffin: LDB3 Antibody [NBP2-19343] - Human lung adenocarcinoma, using LDB3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: LDB3 Antibody [NBP2-19343]

Western Blot: LDB3 Antibody [NBP2-19343]

Western Blot: LDB3 Antibody [NBP2-19343] - Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE gel, diluted at 1:1000.
LDB3 Antibody

Western Blot: LDB3 Antibody [NBP2-19343] -

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LDB3 antibody (NBP2-19343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
LDB3 Antibody

Western Blot: LDB3 Antibody [NBP2-19343] -

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LDB3 antibody (NBP2-19343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
LDB3 Antibody

Western Blot: LDB3 Antibody [NBP2-19343] -

LDB3 antibody detects LDB3 protein by western blot analysis.
A. 30 ug RD whole cell lysate/extract
12% SDS-PAGE
LDB3 antibody (NBP2-19343) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for LDB3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LDB3

This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1 and 2) have C-terminal LIM domains. [provided by RefSeq]

Alternate Names

CMD1C, CYPHER, KIAA01613, KIAA0613FLJ35865, LDB3Z1, LDB3Z4, LIM domain binding 3, LIM domain-binding protein 3, ORACLE, PDLIM6, PDZ and LIM domain 6, Protein cypher, ZASPLVNC3, Z-band alternatively spliced PDZ-motif protein

Gene Symbol

LDB3

UniProt

O75112 (Human)

Additional LDB3 Products

Product Documents for LDB3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LDB3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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