LEO1 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-16704
![Western Blot: LEO1 AntibodyAzide and BSA Free [NBP3-16704]](https://resources.rndsystems.com/images/products/LEO1-Antibody-Western-Blot-NBP3-16704-img0006.jpg "Western Blot: LEO1 AntibodyAzide and BSA Free [NBP3-16704]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 340-570 of human LEO1 (NP_620147.1). ENGLPQDQQEEEPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LEO1 Antibody - Azide and BSA Free
Western Blot: LEO1 AntibodyAzide and BSA Free [NBP3-16704]
Western Blot: LEO1 Antibody [NBP3-16704] - Western blot analysis of extracts of various cell lines, using LEO1 antibody (NBP3-16704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704]](https://resources.rndsystems.com/images/products/LEO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16704-img0005.jpg "Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704]")
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704]
Immunocytochemistry/Immunofluorescence: LEO1 Antibody [NBP3-16704] - Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]](https://resources.rndsystems.com/images/products/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP3-16704-img0003.jpg "Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]")
Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]
Immunohistochemistry-Paraffin: LEO1 Antibody [NBP3-16704] - Immunohistochemistry of paraffin-embedded mouse liver using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Western Blot: LEO1 AntibodyAzide and BSA Free [NBP3-16704]](https://resources.rndsystems.com/images/products/LEO1-Antibody-Western-Blot-NBP3-16704-img0007.jpg "Western Blot: LEO1 AntibodyAzide and BSA Free [NBP3-16704]")
Western Blot: LEO1 AntibodyAzide and BSA Free [NBP3-16704]
Western Blot: LEO1 Antibody [NBP3-16704] - Western blot analysis of extracts of various cell lines, using LEO1 antibody (NBP3-16704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704]](https://resources.rndsystems.com/images/products/LEO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16704-img0004.jpg "Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704]")
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704]
Immunocytochemistry/Immunofluorescence: LEO1 Antibody [NBP3-16704] - Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]](https://resources.rndsystems.com/images/products/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP3-16704-img0001.jpg "Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]")
Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]
Immunohistochemistry-Paraffin: LEO1 Antibody [NBP3-16704] - Immunohistochemistry of paraffin-embedded human colon carcinoma using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]](https://resources.rndsystems.com/images/products/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP3-16704-img0002.jpg "Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]")
Immunohistochemistry-Paraffin: LEO1 Antibody - Azide and BSA Free [NBP3-16704]
Immunohistochemistry-Paraffin: LEO1 Antibody [NBP3-16704] - Immunohistochemistry of paraffin-embedded human esophageal cancer using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704] -
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704] - Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704] -
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704] - Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [LEO1] -
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [LEO1] - Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [LEO1] -
Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [LEO1] - Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for LEO1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: LEO1
Alternate Names
Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae), RDLReplicative senescence down-regulated leo1-like protein, RNA polymerase-associated protein LEO1
Gene Symbol
LEO1
Additional LEO1 Products
Product Documents for LEO1 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LEO1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.govCustomer Reviews for LEO1 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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