LETM1 Antibody - BSA Free

Western Blot: LETM1 Antibody [NBP1-33433]

Western Blot: LETM1 Antibody [NBP1-33433] - A431 lysates (Lane A), H1299 lysates (Lane B), Hela cells (Lane C) and Hep G2 lysates (Lane D) using NBP1-33433 at a dilution of 1:1000.

Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-33433]

Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-33433] - HSC3 xenograft, using LETM1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-33433]

Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-33433] - Mouse colon. LETM1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: LETM1 Antibody [NBP1-33433] -

Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LETM1 Antibody [NBP1-33433] -

Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.