Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LIF Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the center of human LIF. The immunogen is located within amino acids 50 - 100 of LIF. Amino Acid Squence: LYYTAQGEPFPNNLDK

Specificity

At least two isoforms of LIF are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit LIF Antibody - BSA Free (NBP1-76554) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-LIF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LIF Antibody - BSA Free

Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Rat Lung Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF Antibody [NBP1-76554] - 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation with Recombinant Protein. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2ug/mL, 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF AntibodyBSA Free [NBP1-76554]

Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
LIF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LIF Antibody - BSA Free [NBP1-76554] -

Immunocytochemistry/ Immunofluorescence: LIF Antibody - BSA Free [NBP1-76554] - Validation of LIF in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for LIF Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Western Blot

1-4 ug/ml
Application Notes
Use in blocking/neutralizing reported in scientific literature (PMID: 26491045).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIF

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

UniProt

NP_002300 (Human)

Additional LIF Products

Product Documents for LIF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LIF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LIF Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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