LIN-28B Antibody (7D7F7)

Novus Biologicals | Catalog # NBP3-16815

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7D7F7 expressed in HEK293
View all LIN-28B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human LIN-28B (Q6ZN17). MAEGGASKGGGEEPGKLPEPAEEESQVLRGTGHCKWFNVRMGFGFISMINREGSPLDIPVDVFVHQSKLFMEGFRSLKEGEPVEFTFKKSSKGLESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVAN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit LIN-28B Antibody (7D7F7) (NBP3-16815) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LIN-28B Antibody (7D7F7)

Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815]

Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815]

Western Blot: LIN-28B Antibody (7D7F7) [NBP3-16815] - Western blot analysis of extracts of various cell lines, using LIN-28B Rabbit mAb (NBP3-16815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815]

Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815]

Immunohistochemistry-Paraffin: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry of paraffin-embedded human placenta using LIN-28B Rabbit mAb (NBP3-16815) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
LIN-28B Antibody (7D7F7)

Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] -

Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
LIN-28B Antibody (7D7F7)

Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] -

Immunohistochemistry: LIN-28B Antibody (7D7F7) [NBP3-16815] - Immunohistochemistry analysis of paraffin-embedded Human seminoma tissue using LIN-28B Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for LIN-28B Antibody (7D7F7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIN-28B

Lin28B acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7),a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminaluridylation of pre-let-7. Urid

Long Name

LIN-28 Homolog B

Alternate Names

CSDD2, Lin-28.2, LIN28B

Gene Symbol

LIN28B

Additional LIN-28B Products

Product Documents for LIN-28B Antibody (7D7F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LIN-28B Antibody (7D7F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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