LIN-28B Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13029
![Western Blot: LIN-28B Antibody [NBP3-13029]](https://beta-resources.rndsystems.com/images/products/LIN-28B-Antibody-Western-Blot-NBP3-13029-img0001.jpg "Western Blot: LIN-28B Antibody [NBP3-13029]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human LIN-28B. The exact sequence is proprietary.
Reactivity Notes
Sheep (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for LIN-28B Antibody - BSA Free
Western Blot: LIN-28B Antibody [NBP3-13029]
Western Blot: LIN-28B Antibody [NBP3-13029] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN-28B antibody (NBP3-13029) diluted at 1:1000.![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029]](https://beta-resources.rndsystems.com/images/products/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP3-13029-img0003.jpg "Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029]")
Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029]
Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029] - LIN-28B antibody detects LIN-28B protein at cytoplasm and nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. LIN-28B antibody (NBP3-13029) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029]](https://beta-resources.rndsystems.com/images/products/LIN-28B-Antibody-Immunohistochemistry-Paraffin-NBP3-13029-img0002.jpg "Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029]")
Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029]
Immunohistochemistry-Paraffin: LIN-28B Antibody [NBP3-13029] - LIN-28B antibody detects LIN-28B protein at cytoplasm and nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. LIN-28B antibody (NBP3-13029) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for LIN-28B Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LIN-28B
Long Name
LIN-28 Homolog B
Alternate Names
CSDD2, Lin-28.2, LIN28B
Gene Symbol
LIN28B
Additional LIN-28B Products
Product Documents for LIN-28B Antibody - BSA Free
Product Specific Notices for LIN-28B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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