LINE-1 ORF1p Antibody

Novus Biologicals | Catalog # NBP2-66934

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Synthetic peptide within mouse LINE-1 ORF1p aa 308-357 / 357. (SwissProt: P11260 Mouse)

Localization

Cell membrane, nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LINE-1 ORF1p Antibody

Western Blot: LINE-1 ORF1p Antibody [NBP2-66934]

Western Blot: LINE-1 ORF1p Antibody [NBP2-66934]

Western Blot: LINE-1 ORF1p Antibody [NBP2-66934] - Analysis on different tissue lysates using anti- L1ORF1p rabbit polyclonal antibody.
Immunocytochemistry/ Immunofluorescence: LINE-1 ORF1p Antibody [NBP2-66934]

Immunocytochemistry/ Immunofluorescence: LINE-1 ORF1p Antibody [NBP2-66934]

Immunocytochemistry/Immunofluorescence: LINE-1 ORF1p Antibody [NBP2-66934] - Staining L1ORF1p in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications for LINE-1 ORF1p Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

Validated from a verified customer review

Western Blot

1:1000

Reviewed Applications

Read 2 reviews rated 1 using NBP2-66934 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LINE-1 ORF1p

Alternate Names

L LINE-1 retrotransposable element ORF1 protein, L1-ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1, Transposase element L1Md-A101/L1Md-A102/L1Md-A2

Additional LINE-1 ORF1p Products

Product Documents for LINE-1 ORF1p Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LINE-1 ORF1p Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LINE-1 ORF1p Antibody

Customer Reviews for LINE-1 ORF1p Antibody (2)

1 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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2 Stars
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1 Stars
100%

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Customer Images


Showing  1 - 22 reviews Showing All
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  • LINE-1 ORF1p Antibody
    Name: Nesha Rubin
    Application: Immunocytochemistry
    Sample Tested: human ipsc-derived microglia
    Species: Human
    Verified Customer | Posted 03/12/2026
    Microglia staining for ORF1P was non-specific and we confirmed that this was not due to background signal. We have previously also had issues with this antibody staining only the nuclei of SH-SY5Y cells and iPSCs.
    LINE-1 ORF1p Antibody NBP2-66934
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • LINE-1 ORF1p Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Testis
    Species: Mouse
    Verified Customer | Posted 01/09/2021
    ORF1p should be mainly expressed in the cytoplasm of germ cells in testis. This antibody is expressed everywhere, and it's mainly in nuclei. It’s not appropriate for immunofluorescence.
    LINE-1 ORF1p Antibody NBP2-66934
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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FAQs

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