Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) - BSA Free

Novus Biologicals | Catalog # NBP2-52969

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1129

Format

BSA Free
View all Lysine (K)-specific Demethylase 5B/KDM5B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9UGL1, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (86%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of MCF7 cells using the Anti-KDM5B monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human uterus shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human breast cancer shows strong nuclear immunoreactivity in tumor cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human colorectal cancer shows moderate immunoreactivity in cancer cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human liver shows absence of immunoreactivity in hepatocytes (negative control).
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human prostate shows moderate immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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