Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-75964

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysyl Oxidase Homolog 3/LOXL3 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human LOXL3 protein (amino acids 26-753) [UniProt P58215]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free [NBP2-75964]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free [NBP2-75964]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 3/LOXL3 Antibody [NBP2-75964] - Lysyl Oxidase Homolog 3/LOXL3 [NBP2-75964] - IHC analysis of a formalin fixed paraffin embedded tissue section of human small intestine using 1:200 dilution of LOXL3 antibody (NBP2-75964). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated cytoplasm and membrane staining of glandular cells.
Western Blot: Lysyl Oxidase Homolog 3/LOXL3 AntibodyBSA Free [NBP2-75964]

Western Blot: Lysyl Oxidase Homolog 3/LOXL3 AntibodyBSA Free [NBP2-75964]

Western Blot: Lysyl Oxidase Homolog 3/LOXL3 Antibody [NBP2-75964] - Total protein from human A431, HepG2, mouse Neuro2A and rat PC12 cell lines was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LOXL3 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free [NBP2-75964]

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free [NBP2-75964]

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 3/LOXL3 Antibody [NBP2-75964] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-LOXL3 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase Homolog 3/LOXL3

Lysyl oxidase-like protein 3 (LOXL3) is a secreted copper amine oxidase. The N-terminal region of LOXL3 contains four scavenger receptor cysteine-rich (SRCR) domains. The C-terminal region consists of a catalytic domain similar to other lysyl oxidases and a cytokine receptor-like domain. The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone cofactor. LOXL3 is expressed primarily in epidermis, kidney, aorta, and chondrocytes. The enzyme is expressed at low levels in most other tissues. Like lysyl oxidase, LOXL3 oxidizes the lysine e-amino groups of a number of collagen substrates, and is also active against other primary amines. However, LOXL3 does not require proteolytic processing to be activated.

Alternate Names

LOL3, LOXL3

Gene Symbol

LOXL3

Additional Lysyl Oxidase Homolog 3/LOXL3 Products

Product Documents for Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Lysyl Oxidase Homolog 3/LOXL3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

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Protocols

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