MAD1L1/MAD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32775

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Marsupial

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAD1L1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAD1L1/MAD1. The exact sequence is proprietary.

Reactivity Notes

Potorous tridactylus/Potoroo (100%), Bovine (86%).

Localization

Nucleus, Kinetochore

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAD1L1/MAD1 Antibody - BSA Free

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.
Immunocytochemistry/ Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775]

Immunocytochemistry/ Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775]

Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775]

Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775]

Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:10000
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat 2 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775]

Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP1-32775]

Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP1-32775]

Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP1-32775] - Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 ug of MAD1 antibody. Western blot analysis was performed using MAD1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for MAD1L1/MAD1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAD1L1

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Long Name

MAD1 Mitotic Arrest Deficient-Like 1

Alternate Names

HsMAD1, MAD1, PIG9, TP53I9, TXBP181

Gene Symbol

MAD1L1

Additional MAD1L1 Products

Product Documents for MAD1L1/MAD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAD1L1/MAD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAD1L1/MAD1 Antibody - BSA Free

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Protocols

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FAQs

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