MafA Antibody

Novus Biologicals | Catalog # NBP2-24636

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Opossum

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), ICC/IF (Negative)

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MafA Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 300-350 of human MafA was used as the immunogen.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MafA Antibody

Western Blot: MafA Antibody [NBP2-24636]

Western Blot: MafA Antibody [NBP2-24636]

Western Blot: MafA Antibody [NBP2-24636] - Analysis of MafA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using this antibody. 25 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for MafA Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 24509877)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24509877)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24509877)

Western Blot

0.25-0.5ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MafA

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Alternate Names

hMafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1, transcription factor MafA, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)

Entrez Gene IDs

389692 (Human); 378435 (Mouse); 366949 (Rat); 395881 (Chicken)

Gene Symbol

MAFA

UniProt

Q8NHW3 (Human)

Additional MafA Products

Product Documents for MafA Antibody

Certificate of Analysis

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Product Specific Notices for MafA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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