Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C4

Format

BSA Free
View all MAP2 Primary Antibodies »

Product Specifications

Immunogen

MAP2 Antibody (2C4) was developed against full length recombinant human MAP2D. [UniProt# P11137]

Localization

Cytoskeleton

Specificity

This antibody will react with all known MAP2 isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

199 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse MAP2 Antibody (2C4) - BSA Free (NBP2-50032) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MAP2 Antibody (2C4) - BSA Free

Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032]

Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032]

Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Mixed neuron and glia cultures stained with NBP2-50032 (red) and chicken antibody to Vimentin (green) and DNA (blue). NBP2-50032 reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while Vimentin was visualized in astrocytes and fibroblasts.
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032]

Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032]

Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of adult rat hippocampus section stained with mouse mAb to MAP2A/B/C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to FOX2, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. NBP2-50032 antibody labels all isotypes of the MAP2 protein which are found in the perikarya and dendrites of neurons. The FOX2 antibody stains the nuclei of a subset of neuronal cells.
Western Blot: MAP2 Antibody (2C4) [NBP2-50032]

Western Blot: MAP2 Antibody (2C4) [NBP2-50032]

Western Blot: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of tissue and cell lysates using mouse mAb to MAP2C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to Vimentin, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH/3T3 cell lysates. A band at about 280 kDa corresponds to full length intact MAP2A/2B isotypes. The bands that appear at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely to be in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. Vimentin antibody binds to vimentin protein showing a single band at ~50 kDa, which is present in all preparations.
MAP2 Antibody (2C4) - BSA Free

Detection of MAP2 in Mouse Hippocampus via seqIF™ staining on COMET™

MAP2 was detected in immersion fixed paraffin-embedded sections of mouse Hippocampus using Mouse Anti-Human Mouse MAP2, Monoclonal Antibody (Catalog # NBP2-50032) at 1:12,000 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.​​

Applications for MAP2 Antibody (2C4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:5000 - 1:10000

Immunohistochemistry

1:5000 - 1:10000

Western Blot

1:5000 - 1:10000
Application Notes
In Western blot, the strong bands running at about 280 kDa corresponds to MAP2A/B and bands running at 70 kDa correspond to MAP2C/D. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP2

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Long Name

Microtubule-associated Protein 2

Alternate Names

DKFZp686I2148, MAP-2, MAP2A, MAP2B, MAP2C, Microtubule Associated Protein 2, microtubule-associated protein 2, MTAP2

Gene Symbol

MAP2

UniProt

P11137

Additional MAP2 Products

Product Documents for MAP2 Antibody (2C4) - BSA Free

Certificate of Analysis

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Product Specific Notices for MAP2 Antibody (2C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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